Journal: iScience
Article Title: Nicotinamide mononucleotide modified CeO 2 hydrogels promote diabetic wound healing by managing exudate and reducing inflammation
doi: 10.1016/j.isci.2025.114247
Figure Lengend Snippet: Mechanisms underlying the therapeutic effects of CeO 2 @NMN/SA aerogel in promoting diabetic wound healing (A) NAD + levels in HUVEC and HaCat cells following treatment. Data were presented as mean ± SD ( n = 3), ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001. (B) Western blot analysis of Sirt1, Nrf2, and HO-1 protein expression in HaCat and HUVEC cells after treatment. (C–E) Quantitative analysis of Sirt1(C), Nrf2(D), and HO-1(E) protein expression by Western blot in HaCat cells, n = 3. Data were presented as mean ± SD, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001. (F) Representative images of in vitro angiogenesis assays assessing the effects of the gel treatments. Scale bars = 1,000 μm. (G) Statistical analysis of angiogenesis is shown in (G). Mean ± SD, n = 3 ∗∗ p ≤ 0.01, ∗∗∗∗ p ≤ 0.0001. (H) Microscopic images from in vitro migration assays to assess cell migration in response to gel treatment. Scale bars = 1,000 μm. (I) Quantification of cell migration in (H). Data were presented as mean ± SD, ∗ p ≤ 0.05, ∗∗∗ p ≤ 0.001∗∗∗∗ p ≤ 0.0001, n = 3. (J) Evaluation of the antioxidant properties of the gel with and without EX527 treatment, mean ± SD, ∗∗ p ≤ 0.01 n = 3. (K and L) ELISA measurement of inflammatory cytokine expression: IL-6 (K) and IL-10 (L), following gel treatment with and without EX527. Data were presented as mean ± SD, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001, n = 3.
Article Snippet: Heme Oxygenase 1 (HO-1) Rabbit Polyclonal Antibody , HUABIO , RRID: AB_3069172.
Techniques: Western Blot, Expressing, In Vitro, Migration, Enzyme-linked Immunosorbent Assay